Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 3

Research Article

Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities

Figure 3

The relative Th2 cytokines (a) and Th1 cytokines (b) productions from primary T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. Isolated CD4+ helper T cells ( cells/mL) from the DO11.10 transgenic mice with high percentage of OVA peptide-specific T cell receptor were cultured with different doses of Gt-TRE or Gt-PS in the presence of OVA peptide and APC for 72 hrs. The supernatants were collected and cytokine levels were determined by ELISA method. The results are expressed as the mean ± SD of four independent experiments. as compared to the 0 μg/mL control cells.
(a) Th2 cytokines production
(b) Th1 cytokine production

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