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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 764251, 10 pages
http://dx.doi.org/10.1155/2015/764251
Research Article

Protective Effects of N-Acetyl-L-Cysteine in Human Oligodendrocyte Progenitor Cells and Restoration of Motor Function in Neonatal Rats with Hypoxic-Ischemic Encephalopathy

1College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 362-763, Republic of Korea
2Ajou University School of Medicine, Suwon, Gyeonggi 443-380, Republic of Korea
3Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 362-763, Republic of Korea
4Department of Pediatrics, Soonchunhyang University Hospital, Seoul 140-743, Republic of Korea
5Central Research Institute, Dr. Chung’s Food Co. Ltd., Cheongju, Chungbuk 361-782, Republic of Korea
6Division of Neurology, University of British Columbia Hospital, Vancouver, BC, Canada V6T 2B5
7Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Gangwon 210-702, Republic of Korea

Received 16 December 2014; Revised 15 March 2015; Accepted 16 March 2015

Academic Editor: Thomas Lundeberg

Copyright © 2015 Dongsun Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Since oligodendrocyte progenitor cells (OPCs) are the target cells of neonatal hypoxic-ischemic encephalopathy (HIE), the present study was aimed at investigating the protective effects of N-acetyl-L-cysteine (NAC), a well-known antioxidant and precursor of glutathione, in OPCs as well as in neonatal rats. Methods. In in vitro study, protective effects of NAC on KCN cytotoxicity in F3.Olig2 OPCs were investigated via MTT assay and apoptotic signal analysis. In in vivo study, NAC was administered to rats with HIE induced by hypoxia-ischemia surgery at postnatal day 7, and their motor functions and white matter demyelination were analyzed. Results. NAC decreased KCN cytotoxicity in F3.Olig2 cells and especially suppressed apoptosis by regulating Bcl2 and p-ERK. Administration of NAC recovered motor functions such as the using ratio of forelimb contralateral to the injured brain, locomotor activity, and rotarod performance of neonatal HIE animals. It was also confirmed that NAC attenuated demyelination in the corpus callosum, a white matter region vulnerable to HIE. Conclusion. The results indicate that NAC exerts neuroprotective effects in vitro and in vivo by preserving OPCs, via regulation of antiapoptotic signaling, and that F3.Olig2 human OPCs could be a good tool for screening of candidates for demyelinating diseases.