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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 785193, 7 pages
http://dx.doi.org/10.1155/2015/785193
Research Article

Different Proportions of Huangqi (Radix Astragali Mongolici) and Honghua (Flos Carthami) Injection on -Glucosidase and -Amylase Activities

1Department of Pharmacy, Shanxi Provincial People’s Hospital, Taiyuan 030012, China
2Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama University, Toyama 930-0194, Japan

Received 23 September 2014; Accepted 3 March 2015

Academic Editor: Adeolu Alex Adedapo

Copyright © 2015 Hui Liao and Linda Banbury. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To study the effect of different proportions of Huangqi (Radix Astragali Mongolici) and Honghua (Flos Carthami) injection on α-glucosidase and α-amylase activity simultaneously. Methods. The injections were prepared according to the standards of the China Food and Drug Administration. The assay for potential α-glucosidase inhibitors was based on the hydrolysis of 4-methylumbelliferyl-α-D-glucopyranoside (4-MUG). The α-amylase EnzChek assay kit was used to determine potential α-amylase inhibitors. Acarbose was the positive control. Results. The half maximal (50%) inhibitory concentration (IC50) of acarbose against α-glucosidase and α-amylase was () μg/mL and () μg/mL, respectively. Honghua showed significant inhibition of α-glucosidase activity compared with Huangqi (). Honghua inhibited α-amylase activity, but Huangqi did not. IC50s for α-glucosidase inhibition by mixtures at 10 : 1, 5 : 1, and 2 : 1 were significantly lower than those at the 20 : 1 mixture (). α-Amylase inhibition by the 2 : 1 mixture was significantly higher than that by the 20 : 1, 10 : 1, and 5 : 1 mixtures at 500 μg/mL and 1000 μg/mL (), with 5 : 1 significantly higher than 20 : 1 and 10 : 1 at 1000 μg/mL (). Conclusion. Honghua significantly inhibited α-glucosidase activity compared with Huangqi (). For simultaneous inhibition of α-glucosidase and α-amylase activities, the mixtures at 2 : 1 and 5 : 1 exhibited significant effects compared with those at 20 : 1 ().