UP3005, a Botanical Composition Containing Two Standardized Extracts of Uncaria gambir and Morus alba, Improves Pain Sensitivity and Cartilage Degradations in Monosodium Iodoacetate-Induced Rat OA Disease Model

Table 1

Possible synergistic activity of U. gambir and M. alba in protection in composition UP3005.

Sample	Dose (g/mL)	% inhibition	Remark
UP3005	200	71.8	Expected value
		88.5	Experimental value
U. gambir	100	32.6	—
M. alba	100	58.2	—

Rabbit cartilage explants were cultured for 24 hours with rhIL-1α (5 ng/mL) in the absence or presence of a gambir extract, Morus extract, or UP3005 at the disclosed doses to examine the potential protective effect on proteoglycan degradation. Whether a synergistic effect was present was calculated by using Colby’s formula [39]. Expected value: theoretically calculated inhibition value based on Colby’s formula for synergy; experimental value: inhibition observed at 200 g/mL of UP3005; synergy = experimental value ≥ expected value.