The Modified JiuWei QiangHuo Decoction Alleviated Severe Lung Injury Induced by H1N1 Influenza Virus by Regulating the NF-κB Pathway in Mice

Figure 7

MJWQH inhibited the NF-κB signaling pathway. BALB/c mice ( mice/group) were treated as described in Figure 2 and killed on day 2 or 4 after infection. Cytosolic fractions and nuclear extracts were prepared from lung homogenates. NF-κB p65, IκB-α, TLR7, MyD88, and TRAF6 levels were detected by specific antibodies in cytosolic fractions using Western blot analysis. Phosphorylation of IKKα/β and p65 was determined in the nuclear extracts. GAPDH protein was used as a loading control.