Transcriptional Regulation of CYP3A4/2B6/2C9 Mediated via Nuclear Receptor PXR by Helicid and Its Metabolites

Table 1

Primer table of vectors construction.

Primers name	Sequence (5′→3′)
hPXR-F	CTGGAATTCGCCACCATGGAGGTGAGACCCAAAGAAAGC
hPXR-R	CTGGGATCCACGCTACCTGTGATGCCGAACAAC
3A4-1-F	TATGCTAGCGCTGGTTGCTGGTTTATTC
3A4-1-R	TATAGATCTTCTCGTCAACAGGTTAAAGG
3A4-2-F	ATTGCTGGCTGAGGTGGTTG
3A4-2-R	ATATAAGCTTCTGTGTTGCTCTTTGCTG
2B6-1-F	CTGGGTACCCTTTCTCCATCCACAAAATGG
2B6-1-R	CTGCTCGAGGATGCTGATTCAGGGAATGGA
2B6-2-F	CTGAGATCTCTGCAATGAGCACCCAATCTTA
2B6-2-R	CTGAAGCTTCTGCACCCTGCTGCAGCCT
2C9-1-F	TGGGTACCCCATAGAATGTACAACACAAAG
2C9-1-R	CTAGTGAGGTTATTTCCATTTCT
2C9-2-F	CAAAAGGGACATGAGGTGT
2C9-2-R	CTTGAAGCTTTCTCTTCTTGTTAAGACAACCA

Boldfaces for the restriction sites.