Effect of Keishibukuryogan on Genetic and Dietary Obesity Models

Figure 3

Effect of KBG on serum levels of leptin and insulin in obesity models. (a, b) SHR rats were administered with control water or KBG (500 mg/kg, p.o., daily) for 8 weeks. Serum samples were collected upon the termination and levels of leptin (left) or insulin (right) were measured. (b, c) C57BL/6 mice were fed with normal diet (ND) or high-fat diet (HFD) for 10 weeks and then administered with control water or KBG (500 mg/kg, p.o., daily) for 12 weeks under the same feeding condition. After 8 weeks of control or KBG treatment, mice fed with HFD were changed to ND until the termination of experiment with maintaining KBG treatment for another 4 weeks. Serum samples were collected before the time for food changing (b) or upon the termination (c) and levels of leptin (left) or insulin (right) were measured by using specific ELISA assay. Data are mean ± SEM ( = 7–15). ; .

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