Research Article

[Retracted] Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro

Figure 3

Detection of oocyte markers in OLCs by immunofluorescent analysis. (a), (b), and (c) show immunofluorescent stains of SP1, SCP3, GDF9, and ZP1 or ZP2 in OLCs on day 24 after treatment with the rat medicated serums containing common ingredients of ZGW/YGW, YGW, and ZGW, respectively. (d) shows the immunofluorescent analysis of expressions of SP1, SCP3, GDF9, and ZP1 in stems cells treated with normal rat serum. (e) shows negative controls obtained by incubating the stems cells with secondary anti-rabbit or anti-mouse antibody alone after staining with DAPI. Scale bar is set to 50 μm.
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