The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/β-Catenin Signaling Activation in Podocytes

Figure 5

Effects of curcumin on podocyte-associated molecules of mice. (a) Mice sacrificed at the end of 12th week. Total RNA was extracted from kidney cortex, and then the relative mRNA expression levels of nephrin, podocin, podoplanin, and podocalyxin were measured by relative quantitative real-time RT-PCR. (b) Kidney cortex tissue was lysed and total lysates were analyzed by Western blot assay with antibodies of nephrin, podocin, podoplanin, podocalyxin, and β-actin antibody, respectively. The relative protein level was expressed as the protein/β-actin ratio. Values are represented as mean ± SD (). versus control group, versus control group, and versus model group.

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