The Protective Effects of Curcumin on Obesity-Related Glomerulopathy Are Associated with Inhibition of Wnt/β-Catenin Signaling Activation in Podocytes

Figure 8

Effects of DKK1 on leptin-mediated podocyte injury. Well-differentiated conditionally immortalized mouse podocytes were incubated in normal media, media containing 15 ng/mL leptin and medium containing both 15 ng/mL leptin and 200 ng/mL DKK1, a well-known inhibitor of Wnt/β-catenin signaling, respectively. (a) After 9 h, cells were collected and the mRNA expression levels of nephrin, podocin, podoplanin, and podocalyxin were measured by relative quantitative real-time RT-PCR. (b) After 24 h, cells were lysed and total lysates were analyzed by Western blot assay with antibodies of nephrin, podocin, podoplanin, podocalyxin, and β-actin, respectively. The relative protein level was expressed as the protein/β-actin ratio. Values are represented as mean ± SD (). versus control group, versus control group, and versus leptin alone group.

(a)

(b)