Bacopa monniera (CDRI-08) Upregulates the Expression of Neuronal and Glial Plasticity Markers in the Brain of Scopolamine Induced Amnesic Mice

Table 1

Primer sequences and PCR conditions.

Gene	Sequence of forward and reverse primers (5′→3′)	Condition of; (a) denaturation; (b) annealing; (c) extension	Number of cycles
Arc [17]	FP-GGCGACCAGATGGAGCTGGACCATA-; RP-CTGGCCCCTCTATTCAGGCTGGGTC-	(a) 94°C, 1 min; (b) 59°C, 1 min; (c) 72°C, 1.5 min	35
BDNF [20]	FP-TGCCAGAGCCCCAGGTGTGA-; RP-CTGCCCTGGGCCCATTCACG-	(a) 94°C, 1 min; (b) 63°C, 30 sec; (c) 72°C, 45 sec	32
GFAP [20]	FP-TTCCTGTACAGACTTCTCC-; RP-CCCTTCAGGACTGCCTTAGT-	(a) 94°C, 1 min; (b) 52°C, 30 sec; (c) 72°C, 45 sec	29
GAPDH [20]	FP-GTCTCCTGCGACTTCAG-; RP-TCATTGTCATACCAGGAAATGAGC-	(a) 94°C, 1 min; (b) 52°C, 30 sec; (c) 72°C, 30 sec	26