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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 839697, 9 pages
http://dx.doi.org/10.1155/2015/839697
Research Article

Immunoregulation on Mice of Low Immunity and Effects on Five Kinds of Human Cancer Cells of Panax japonicus Polysaccharide

1Third Xiangya Hospital, Central South University, Changsha 410013, China
2Changsha University, Changsha 410022, China
3School of Pharmaceutical Science, Central South University, Changsha 410013, China
4The Central Hospital of Taian, Taian 271000, China
5Maternal and Child Health Hospital, Liuyang 410300, China

Received 25 December 2014; Revised 9 April 2015; Accepted 16 April 2015

Academic Editor: Jairo Kennup Bastos

Copyright © 2015 Zhang Jie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The goal of this study is to investigate the immunoregulative effects of Panax japonicus polysaccharide (PJPS) on mice of low immunity. An orthogonal experiment was designed to determine the best extraction process for PJPS. By the tests of macrophages swallow chicken red blood cells, Delayed-type hypersensitivity (DTH), and serum hemolysin value, we studied the immune adjustment ability of PJPS. MTT was employed to detect the effects of different concentrations of PJPS, respectively, in 24 h, 48 h, and 72 h on five kinds of human cancer cells. The results show that the best extraction process for PJPS was as follows: ratio of solvent consumption to raw material 40, extraction temperature 100°C, re-extracted two times, each extraction time 4 hours. PJPS can significantly improve the immune function of mice processed by cyclophosphamide and PJPS did not work on the above five cancer cells.