Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species

Figure 5

Gambogic acid lysinate can induce MCF-7 cells apoptosis by SIRT1 and caspase-3 signal pathway. MCF-7 cells were treated with the indicated concentrations of GAL for 24 h, and the cell extracts were prepared and analyzed by Western blotting with corresponding antibodies. The blots were quantified by densitometry with Scion Image (Scion Corporation, Frederick, MD, USA), and the relative ratio of target protein toβ-actin was calculated and expressed as the mean ± SD from three experiments. versus the control group.