Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 863531, 10 pages
http://dx.doi.org/10.1155/2015/863531
Research Article

Protective Effect and Mechanism of Total Flavones from Rhododendron simsii Planch Flower on Cultured Rat Cardiomyocytes with Anoxia and Reoxygenation

1Department of Pharmacology, Anhui Medical University, Hefei, Anhui 230032, China
2Xinglin College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110167, China

Received 14 August 2014; Revised 25 December 2014; Accepted 31 December 2014

Academic Editor: Hyunsu Bae

Copyright © 2015 Yi Jiao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Kambe, R. Bessho, M. Fujii, M. Ochi, and K. Shimizu, “Sivelestat reduces myocardial ischemia and reperfusion injury in rat hearts even when administered after onset of myocardial ischemia,” Interactive Cardiovascular and Thoracic Surgery, vol. 8, no. 6, pp. 629–634, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. M. Dong, B. P. Yan, J. K. Liao, Y.-Y. Lam, G. W. K. Yip, and C.-M. Yu, “Rho-kinase inhibition: a novel therapeutic target for the treatment of cardiovascular diseases,” Drug Discovery Today, vol. 15, no. 15-16, pp. 622–629, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. T. Hattori, H. Shimokawa, M. Higashi et al., “Long-term inhibition of rho-kinase suppresses left ventricular remodeling after myocardial infarction in mice,” Circulation, vol. 109, no. 18, pp. 2234–2239, 2004. View at Publisher · View at Google Scholar · View at Scopus
  4. S. A. Hamid, H. S. Bower, and G. F. Baxter, “Rho kinase activation plays a major role as a mediator of irreversible injury in reperfused myocardium,” The American Journal of Physiology—Heart and Circulatory Physiology, vol. 292, no. 6, pp. H2598–H2606, 2007. View at Publisher · View at Google Scholar · View at Scopus
  5. W. Bao, E. Hu, L. Tao et al., “Inhibition of Rho-kinase protects the heart against ischemia/reperfusion injury,” Cardiovascular Research, vol. 61, no. 3, pp. 548–558, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. T. Sato, B. O'Rourke, and E. Marbán, “Modulation of mitochondrial ATP-dependent K+ channels by protein kinase C,” Circulation Research, vol. 83, no. 1, pp. 110–114, 1998. View at Publisher · View at Google Scholar · View at Scopus
  7. R.-H. Du, T. Dai, W.-J. Cao, M. Lu, J.-H. Ding, and G. Hu, “Kir6.2-containing ATP-sensitive K+ channel is required for cardioprotection of resveratrol in mice,” Cardiovascular Diabetology, vol. 13, no. 1, article 35, 2014. View at Publisher · View at Google Scholar · View at Scopus
  8. J. C. Cleveland Jr., D. R. Meldrum, R. T. Rowland, A. Banerjee, and A. H. Harken, “Adenosine preconditioning of human myocardium is dependent upon the ATP-sensitive K+ channel,” Journal of Molecular and Cellular Cardiology, vol. 29, no. 1, pp. 175–182, 1997. View at Publisher · View at Google Scholar · View at Scopus
  9. R. J. Diaz, C. Zobel, H. C. Cho et al., “Selective inhibition of inward rectifier k+ channels (kir2.1 or kir2.2) abolishes protection by ischemic preconditioning in rabbit ventricular cardiomyocytes,” Circulation Research, vol. 95, no. 3, pp. 325–332, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. J.-H. Zhang, Z.-W. Chen, and Z. Wu, “Late protective effect of pharmacological preconditioning with total flavones of rhododendra against myocardial ischemia-reperfusion injury,” Canadian Journal of Physiology and Pharmacology, vol. 86, no. 3, pp. 131–138, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. L. P. Yuan, Z. W. Chen, F. Li, L. Y. Dong, and F. H. Chen, “Protective effect of total flavones of rhododendra on ischemic myocardial injury in rabbits,” The American Journal of Chinese Medicine, vol. 34, no. 3, pp. 483–492, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. J. Han, G.-W. He, and Z.-W. Chen, “Protective effect and mechanism of total flavones from rhododendron simsii planch on endothelium-dependent dilatation and hyperpolarization in cerebral ischemia-reperfusion and correlation to hydrogen sulphide release in rats,” Evidence-Based Complementary and Alternative Medicine, vol. 2014, Article ID 904019, 11 pages, 2014. View at Publisher · View at Google Scholar
  13. S.-J. Dai, R.-Y. Chen, and D.-Q. Yu, “Studies on the flavonoid compounds of Rhododendron anthopogonoides,” China Journal of Chinese Materia Medica, vol. 29, no. 1, pp. 44–47, 2004. View at Google Scholar · View at Scopus
  14. Y. Huang, P. Yin, D. F. Jiang et al., “Quality standard of rhododendron flos,” World Science and Technology, vol. 16, no. 1, pp. 151–155, 2014. View at Google Scholar
  15. E. Missov, C. Calzolari, and B. Pau, “Circulating cardiac troponin I in severe congestive heart failure,” Circulation, vol. 96, no. 9, pp. 2953–2958, 1997. View at Publisher · View at Google Scholar · View at Scopus
  16. D.-J. Sung, J.-G. Kim, K. J. Won et al., “Blockade of K+ and Ca2+ channels by azole antifungal agents in neonatal rat ventricular myocytes,” Biological and Pharmaceutical Bulletin, vol. 35, no. 9, pp. 1469–1475, 2012. View at Google Scholar · View at Scopus
  17. Y. Yan, O. Wuliji, X. Zhao et al., “Effect of essential oil of Syringa pinnatifolia Hemsl. var. alashanensis on ischemia of myocardium, hypoxia and platelet aggregation,” Journal of Ethnopharmacology, vol. 131, no. 2, pp. 248–255, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. T. Omland, J. A. De Lemos, M. S. Sabatine et al., “A sensitive cardiac troponin T assay in stable coronary artery disease,” The New England Journal of Medicine, vol. 361, no. 26, pp. 2538–2547, 2009. View at Publisher · View at Google Scholar · View at Scopus
  19. K. Thygesen, J. S. Alpert, A. S. Jaffe et al., “Third universal definition of myocardial infarction,” Circulation, vol. 126, no. 16, pp. 2020–2035, 2012. View at Publisher · View at Google Scholar
  20. Y.-E. Zhang, J.-N. Wang, J.-M. Tang et al., “In Vivo protein transduction: delivery of PEP-1-SOD1 fusion protein into myocardium efficiently protects against ischemic insult,” Molecules and Cells, vol. 27, no. 2, pp. 159–166, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. M. K. Ozer, H. Parlakpinar, N. Vardi, Y. Cigremis, M. Ucar, and A. Acet, “Myocardial ischemia/reperfusion-induced oxidative renal damage in rats: protection by caffeic acid phenethyl ester (Cape),” Shock, vol. 24, no. 1, pp. 97–100, 2005. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Wibberley, Z. Chen, E. Hu, J. P. Hieble, and T. D. Westfall, “Expression and functional role of Rho-kinase in rat urinary bladder smooth muscle,” British Journal of Pharmacology, vol. 138, no. 5, pp. 757–766, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. G. Loirand, P. Guérin, and P. Pacaud, “Rho kinases in cardiovascular physiology and pathophysiology,” Circulation Research, vol. 98, no. 3, pp. 322–334, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. S. L. M. Peters and M. C. Michel, “The RhoA/Rho kinase pathway in the myocardium,” Cardiovascular Research, vol. 75, no. 1, pp. 3–4, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. V. Q. Chau, F. N. Salloum, N. N. Hoke, A. Abbate, and R. C. Kukreja, “Mitigation of the progression of heart failure with sildenafil involves inhibition of RhoA/Rho-kinase pathway,” The American Journal of Physiology—Heart and Circulatory Physiology, vol. 300, no. 6, pp. H2272–H2279, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. A. Yatani, K. Irie, T. Otani, M. Abdellatif, and L. Wei, “RhoA GTPase regulates L-type Ca2+ currents in cardiac myocytes,” The American Journal of Physiology —Heart and Circulatory Physiology, vol. 288, no. 2, pp. H650–H659, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. A. J. McNeish, F. Jimenez-Altayo, G. S. Cottrell, and C. J. Garland, “Statins and selective inhibition of rho kinase protect small conductance calcium-activated potassium channel function (k(ca)2.3) in cerebral arteries,” PLoS ONE, vol. 7, no. 10, Article ID e46735, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. S. Chandra, M. J. Romero, A. Shatanawi, A. M. Alkilany, R. B. Caldwell, and R. W. Caldwell, “Oxidative species increase arginase activity in endothelial cells through the rhoa/rho kinase pathway,” British Journal of Pharmacology, vol. 165, no. 2, pp. 506–519, 2012. View at Publisher · View at Google Scholar · View at Scopus
  29. K. Noma, C. Goto, K. Nishioka et al., “Roles of rho-associated kinase and oxidative stress in the pathogenesis of aortic stiffness,” Journal of the American College of Cardiology, vol. 49, no. 6, pp. 698–705, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. J. Zhang, X.-X. Li, H.-J. Bian, X.-B. Liu, X.-P. Ji, and Y. Zhang, “Inhibition of the activity of Rho-kinase reduces cardiomyocyte apoptosis in heart ischemia/reperfusion via suppressing JNK-mediated AIF translocation,” Clinica Chimica Acta, vol. 401, no. 1-2, pp. 76–80, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. L. Chu, I. Norota, and M. Endoh, “Differential inhibition by the Rho kinase inhibitor Y-27632 of the increases in contractility and Ca2+ transients induced by endothelin-1 in rabbit ventricular myocytes,” Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 371, no. 3, pp. 185–194, 2005. View at Publisher · View at Google Scholar · View at Scopus
  32. Z. W. Chen, C. G. Ma, M. Fang, and S. Y. Xu, “The blocking effect of hyperin on the inward flow of calcium ion,” Yao Xue Xue Bao, vol. 29, no. 1, pp. 15–19, 1994. View at Google Scholar · View at Scopus
  33. L. Stirling, M. R. Williams, and A. D. Morielli, “Dual roles for rhoa/rho-kinase in the regulated trafficking of a voltage-sensitive potassium channel,” Molecular Biology of the Cell, vol. 20, no. 12, pp. 2991–3002, 2009. View at Publisher · View at Google Scholar · View at Scopus
  34. Y. Liu, X.-H. Xu, Z. Liu et al., “Effects of the natural flavone trimethylapigenin on cardiac potassium currents,” Biochemical Pharmacology, vol. 84, no. 4, pp. 498–506, 2012. View at Publisher · View at Google Scholar · View at Scopus