Yokukansan Improves Mechanical Allodynia through the Regulation of Interleukin-6 Expression in the Spinal Cord in Mice with Neuropathic Pain

Figure 2

Effects of yokukansan and the constituent herbal medicines on mechanical allodynia in mice with partial sciatic nerve ligation (PSL). (a) Time schedule of drug administration and the evaluation of mechanical allodynia. Partial sciatic nerve ligation (PSL) was performed on day 0. Mechanical allodynia was tested by punctate stimulation using a von Frey filament with a bending force of 0.16 g applied to the footpad of the paw on the PSL side. Yokukansan extract (YKS, 1 g/kg) (b), a mixture (MIX) of Glycyrrhizae Radix (GR, 100 mg/kg) and Atractylodis Lanceae Rhizoma (ALR, 200 mg/kg) extracts (c), ALR extract (ALR, 200 mg/kg) (d), GR extract (100 mg/kg) (e), or vehicle (VH) was orally administered once a day from day 4 after surgery for 7 days. Data are presented as means ± standard errors of the means (). , compared with the vehicle-treated group (Holm-Sidak test).

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