Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 2

Research Article

Traditional Korean Herbal Formula Samsoeum Attenuates Adipogenesis by Regulating the Phosphorylation of ERK1/2 in 3T3-L1 Cells

Figure 2

Inhibitory effect of SSE extract on triglyceride production in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by incubation with isobutylmethylxanthine, dexamethasone, and insulin (MDI) for 8 days. The cells were treated with or without SSE or GW9662 (20 μM) during the differentiation period. ((a) and (b)) Lipid accumulation in the cells was analyzed by Oil Red O staining. (a) The stained cells were visualized on an Olympus CKX41 inverted microscopy at ×200 of magnification. (b) Stained oil droplets were dissolved in isopropyl alcohol and quantified by reading the absorbance at 520 nm. (c) The triglyceride content was measured enzymatically using a commercial kit (BioVision Inc.) at 570 nm. Data are presented as mean SEM. and versus differentiated cells.