Table 5: MIC of antifungal drugs and effect of combination with coumarin derivative, 7-hydroxy-6-nitro-2H-1-benzopyran-2-one (Cou-1), against A. fumigatus, ATCC 46913.

Antifungal + Cou-UNO2MIC FIC index
(μg/mL)(Type of interaction)

Cou-UNO216
Amphotericin B2
Flucanozole256
Itraconazole128
Voriconazole2
Cou-UNO2/Amphotericin B16/22 (indifferent)
Cou-UNO2/Flucanozole16/2562 (indifferent)
Cou-UNO2/Itraconazole4/640,75 (additivity)
Cou-UNO2/Voriconazole8/0,50,5 (additivity)

Note. Cou-UNO2: 7-hydroxy-6-nitro-2H-1-benzopyran-2-one. FIC: fractional inhibitory concentration. MIC: minimal inhibitory concentration.