Evaluation of Antifungal Activity and Mode of Action of New Coumarin Derivative, 7-Hydroxy-6-nitro-2H-1-benzopyran-2-one, against Aspergillus spp.

Table 5

MIC of antifungal drugs and effect of combination with coumarin derivative, 7-hydroxy-6-nitro-2H-1-benzopyran-2-one (Cou-1), against A. fumigatus, ATCC 46913.

Antifungal + Cou-UNO2	MIC	FIC index
	(μg/mL)	(Type of interaction)
Cou-UNO2	16
Amphotericin B	2
Flucanozole	256
Itraconazole	128
Voriconazole	2
Cou-UNO2/Amphotericin B	16/2	2 (indifferent)
Cou-UNO2/Flucanozole	16/256	2 (indifferent)
Cou-UNO2/Itraconazole	4/64	0,75 (additivity)
Cou-UNO2/Voriconazole	8/0,5	0,5 (additivity)

Note. Cou-UNO₂: 7-hydroxy-6-nitro-2H-1-benzopyran-2-one. FIC: fractional inhibitory concentration. MIC: minimal inhibitory concentration.