Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2/M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways

Figure 3

P2 effectively induced cycle arrest at G2/M phase in HeLa cells. (a) Effect of P2 on cell cycle distribution of HeLa cells. Cells were treated with 0, 4, 12, and 36 μg/mL of P2 for 48 h and stained with PI. DNA contents in each treatment were then determined by FCM. Data showed a representative experiment in triplicate with similar results. (b) Effect of P2 on protein expression of cyclin B1, p-cdc2, and p21 in HeLa cells. Cells were treated with 0, 4, 12, and 36 μg/mL of P2 for 48 h and the protein expression of cyclin B1, p-cdc2, and p21 was detected by western blot assay. GAPDH was used as an internal control. Results shown are the representation of three independent experiments.

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