Polypeptide Fraction from Arca subcrenata Induces Apoptosis and G2/M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways

Figure 4

P2 highly enhanced the intracellular generation of ROS in HeLa cells. (a) Effect of P2 on ROS level in HeLa cells being treated for different time. Cells were treated with 12 μg/mL of P2 for 0, 0.5, 1, 2, 4, 6, and 9 h, respectively, and detected for the fluorescence of DCFH-DA staining by FCM. Compared with the control, (). (b, c) Effect of P2 at different concentrations on ROS level in HeLa cells. Cells were treated with 0, 4, 12, and 36 μg/mL of P2 for 6 h and detected for the fluorescence of DCFH-DA staining. Compared with the control, , ().

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