Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress

Figure 2

Cytofluorimetric evaluation of intracellular ROS. A549 cells treated for 18 h with FFBJ or FFOJ were oxidatively stressed by H₂O₂ 200 μM for additional 2 h. Results from FFBJ (a) or FFOJ (b) treatments. The curves shifted rightward to higher emission values indicate the increase of ROS production. Data from (a) and (b) are expressed as percentage of reduction (%Δ) of DCF-DA emission values in FFBJ or FFOJ-pretreated cells and then exposed to H₂O₂ compared to H₂O₂-stressed cells (c). The experiments were repeated at least three times. versus H₂O₂-treated cells.

(a)

(b)

(c)