Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress

Figure 7

Effect of FFBJ or FFOJ on mitochondrial membrane potential in H₂O₂-treated cells. The cells treated with FFBJ or FFOJ and then exposed to 200 μM H₂O₂ for 2 h were loaded with R123 probe. Fluorescence was followed by flow cytometry. The shift of the curves to the left of the graph indicates the reduction of . Results from FFBJ (a) or FFOJ (b) exposure. In (c) are reported data from (a) and (b) expressed as percentage of increase (%Δ) of R123 emission values in FFBJ- or FFOJ-pretreated cells subsequently exposed to H₂O₂ compared to H₂O₂-stressed cells. and versus H₂O₂-treated cells.

(a)

(b)

(c)