Research Article
Ligustrazine Inhibits Cartilage Endplate Hypertrophy via Suppression of TGF-β1
Figure 2
Effects of LIG on cell proliferation and Col1a and TGF-β expression in vitro. (a) MTT assays showed that passage-III hypertrophic CEP cell proliferation decreased on day 13 after LIG treatment at two doses compared to the control (0 M). The inhibitory effect of LIG was more pronounced at a dose of 10−5 M than at 10−7 M. (b–d) Condensed cells were treated with LIG. (b) Col1a1 expression was significantly decreased by LIG of 10−7 M. LIG also showed inhibitory tendency at a dose of 10−5 M with no statistical significance. (c) Cytoimmunofluorescence results showed attenuated positive staining for Col I in the extracellular matrix with LIG of 10−7 M compared to the nontreatment. (d) Western blot analysis of TGF-β expression by 10 days of LIG treatment at a dose of 10−7 M. Each column represents the mean ± SE of three independent experiments. versus 0 M.
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