Hataedock Treatment Has Preventive Therapeutic Effects in Atopic Dermatitis-Induced NC/Nga Mice under High-Fat Diet Conditions

Figure 7

Upregulation of apoptosis. Upregulation of apoptosis in dermatitis induced by HTD treatment. The apoptotic body (arrow indicates dark brown) in the HTT group was remarkably increased compared to the HDE group (TUNEL assay; square box, enlarged DNA fragmentation of nucleus with TUNEL positive reaction; bar size, 50 μm). The Bcl-2 positive reaction (arrow indicates dark brown) in HTT group was remarkably decreased compared with those of the HDE group (immunohistochemistry; bar size, 50 μm). The cleaved caspase-3 positive reaction (arrow indicates dark brown) in HTT group was increased compared with those of the HDE group (immunohistochemistry; bar size, 50 μm). Data of TUNEL, Bcl-2, and cleaved caspase-3 image analysis was also showing the same result (). Ctrl: normal feeding, HF: high-fat diet, HDE: high-fat diet and untreated AD-induced, HTT: high fat diet and Hataedock treated AD-induced, EP: epidermis, and DE: dermis. , compared with the Ctrl group; , compared with the HF group; , compared with the HDE group.