Sex-Dependent Effects of Dietary Genistein on Echocardiographic Profile and Cardiac GLUT4 Signaling in Mice

Figure 3

Effect of genistein treatment on total cardiac GLUT4 protein expression in intact male and female cardiac tissue. Typical western blot demonstrating GLUT4 and actin expression in cardiac tissue from two samples each of 600 G- and 0 G-treated male and female mice. Average GLUT4/actin ratio is shown comparing 600 G- and 0 G-fed male and female mice (/group). GLUT4 and actin bands were observed at 40–48 kDa and 43 kDa, respectively. The genistein-free fed groups (0 G) are represented by open bars, and genistein-fed groups (600 G) are represented by the solid bars. Values are reported as mean ± SD and denotes sex-dependent difference (). # denotes genistein-mediated difference, . 0 GF: genistein-free fed females; 0 GM: genistein-free fed males; 600 GF: 600 mg genistein/kg diet-fed females; 600 GM: 600 mg genistein/kg diet-fed males; GLUT4: glucose transporter 4.