Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer’s Disease

Figure 1

Chromatographic fingerprint analysis and cytotoxicity assay of the herbal formula B401. (a) Chromatographic fingerprint analysis was conducted by using LC/MS analysis. Fifteen bioactive marker substances from ingredients of the herbal formula B401 were qualitatively determined within 80 min under selected LC/MS condition. Bioactive marker substances for Astragalus membranaceus: calycosin-7-O-β-D-glucoside (peak 1), ononin (peak 2), calycosin (peak 3), formononetin (peak 4), astragaloside (peak 5), isoastragaloside (peak 6), astragaloside II (peak 7), and astragaloside IV (peak 8); Angelica sinensis: Z-ligustilide (peak 9); Rehmannia glutinosa: forsythiaside (peak 10) and acteoside (peak 11); Eclipta prostrata: wedelolactone (peak 12); Ligustri fructus: oleanolic acid (peak 13); Panax ginseng: ginsenoside Rc (peak 14) and ginsenoside Rb2 (peak 15). (b) (A) Cell viability was measured by MTT assay after Tet-On Aβ₄₂-GFP SH-SY5Y cells were treated without (control) and with the herbal formula B401 at indicated doses ( for each treatment). (B) IC₅₀ values of the herbal formula B401 for Tet-On Aβ₄₂-GFP SH-SY5Y cells were reported in the dose-response curve. Results were shown as mean ± SEM, and the number of experiments was six for each treatment.

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