Neuroprotective Effects of the Herbal Formula B401 in Both Cell and Mouse Models of Alzheimer’s Disease

Figure 5

Oral B401 treatment effectively improves subcutaneous microcirculation and enhances brain VEGF expression in 3× Tg-AD mice. (a) (A) Dorsal subcutaneous microcirculatory flow imaging in 3× Tg-AD mice and their control (non-AD mice) by using moorFLPI laser Doppler imager. (B) Quantified dorsal subcutaneous microcirculatory flow in 3× Tg-AD mice with oral B401 treatment was significantly greater compared to those 3× Tg-AD mice with sham treatment. (b) Western blotting analysis shows the following. (A) Whole brain VEGF expression levels of 3× Tg-AD mice and their control. (B) Quantified brain VEGF levels of the 3× Tg-AD mice with oral B401 treatment were significantly greater compared to those 3× Tg-AD mice with sham treatment and their control. Results were shown as mean ± SEM (; , one-way ANOVA followed by a Student-Newman-Keuls multiple comparisons post test), and the number of experiments was six for each treatment.

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