Electroacupuncture Reduces Postoperative Pain and Analgesic Consumption in Patients Undergoing Thoracic Surgery: A Randomized Study

Chen, Tongyu; Wang, Ke; Xu, Jianjun; Ma, Wen; Zhou, Jia

doi:https://doi.org/10.1155/2016/2126416

Evidence-Based Complementary and Alternative Medicine

On this page

Abstract Introduction Materials and Methods Results Discussion Authors’ Contributions Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2016 | Article ID 2126416 | https://doi.org/10.1155/2016/2126416

Electroacupuncture Reduces Postoperative Pain and Analgesic Consumption in Patients Undergoing Thoracic Surgery: A Randomized Study

Tongyu Chen,¹Ke Wang,¹Jianjun Xu,¹Wen Ma,²and Jia Zhou¹

Academic Editor: Shu-Ming Wang

Received05 Dec 2015

Revised15 Feb 2016

Accepted24 Feb 2016

Published17 Mar 2016

Abstract

The aim of this study was to evaluate the effect of electroacupuncture (EA) on postoperative pain management in patients undergoing thoracic surgery. A randomized study was conducted. Ninety-two thoracic surgical patients were randomly divided into an EA group and a sham group. Postoperative intravenous analgesia was applied with a half dose of the conventional drug concentration in both groups. In the EA group, EA treatment was administered for three consecutive days after the surgery with 6 sessions of 30 min each. Compared with the sham group, patients in the EA group had a lower visual analogue scale (VAS) score at 2, 24, 48, and 72 hours and consumed less analgesic after surgery. The incidence of opioid-related adverse effects of nausea was lower in the EA group. The time to first flatus and defecation was also shorter in the EA group. Furthermore, the plasma β-endorphin (β-EP) level was higher by radioimmunoassay and the plasma 5-hydroxytryptamine (5-HT) level was lower in the EA group by enzyme-linked immunosorbent assay during the first 72 hr after thoracic surgery. Therefore, EA is suitable as an adjunct treatment for postoperative pain management after thoracic surgery.

1. Introduction

Thoracotomy can cause significant postoperative pain especially at the first 48 hours after the surgery [1]. Uncontrolled postthoracotomy pain is an independent risk factor for the development of postoperative complications such as fever and respiratory failure [2]. Poorly controlled postthoracotomy pain has led to the development of chronic thoracic syndrome and has a significant negative impact on the quality of life of these patients [3]. Various pain interventions including intravenous analgesia and epidural and peripheral nerve blocks have been used as part of multimodal pain management in caring for patients undergoing thoracotomy [4]. However, despite these well-established pain controlling methods, postthoracotomy pain remains.

Acupuncture has been used for thousands of years for various medical conditions including pain management. Electroacupuncture (EA) is one of the needle stimulation techniques that utilize electricity instead of manual manipulation. EA analgesia has been found to be effective as adjunctive therapy for postoperative pain management in patients that underwent total knee arthroplasty, prostatectomy, and thyroid surgery [5–7]. The use of EA has decreased the pain intensity, the consumption of cumulative opioids, and the side effect associated with the use of opioids. To date, only one prior study utilized EA as part of multimodal pain management for patients undergoing thoracotomy [8]. However, the sample size is too small (only 27 patients) to achieve statistical significant differences. Previous clinical and animal studies have demonstrated that endogenous opioids play a pivotal role in EA analgesia. Low-frequency (2 Hz) EA is highly effective in triggering the release of β-endorphin (β-EP) [9]. EA was also found to affect the release of 5-hydroxytryptamine (5-HT) [10]. Therefore, we designed this clinical trial not only to evaluate whether EA can serve as an adjunct to intravenous analgesic but also to evaluate whether it affects the endogenous β-EP and 5-HT release for patients undergoing small incision thoracotomy. Our primary aim is to determine whether EA can reduce postoperative analgesic requirement over the first 72 hours after surgery, and the secondary aim is to determine whether the incidence of opioid-related side effect (i.e., PONV) is less in EA treatment group than in the sham control group. The blood sample will be collected at 2 hr, 24 hr, 48 hr, and 72 hr after surgery.

2. Materials and Methods

2.1. Research Design and Participants

This study was conducted in Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from January 2012 to July 2014. Ninety-two patients were assigned to either an EA group () or a sham group () using a randomization sequence based on a table of randomly generated numbers. To conceal the allocation, randomization was carried out using sequentially numbered, opaque, sealed envelopes. The trial was approved by the Ethics Committee of the Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine. All participating patients gave signed informed consent before being enrolled in this study.

Patients undergoing small incision lobectomy surgery participated in the study. Inclusion criteria were as follows: age 16–80 years, general anesthesia, and American Society of Anesthesiologists (ASA) grade I-II. Exclusion criteria were as follows: use of antiemetics or morphine before surgery, usage of a pacemaker, history of arrhythmia or epilepsy, opiate dependence, abnormally shaped cutaneous lesions at the application sites, and previous participation in similar experiments (any acupuncture or EA treatment). Figure 1 shows the flow chart of the research protocol and allocation of participants in this study.

2.2. Interventions

The acupuncture treatments were performed by an acupuncturist with 10 years of acupuncture experience. Patients in the EA group received postoperative EA starting 1 hour after surgery. This treatment was repeated every 12 hours for three days after surgery. Patients received a total of six EA treatments. The acupuncture needles (0.25 × 40 mm, Huatuo, Suzhou Medical Instruments Factory, Suzhou, China) were inserted into acupoints, including bilateral Taichong (LR 3), Yang Lingquan (GB 34), Waiguan (TE 5), and Chize (LU 5), which were selected based on traditional Chinese medicine (TCM) meridian theory. The depth of needle insertion was usually 6–10 mm based on the patient’s skin thickness and subcutaneous fatty tissues at the site of the acupuncture points. Next, the performer manipulated the needles until the patient felt a de qi sensation (deep cramp-like sensation). EA stimulation was applied for 30 min using LH202H HANS acupuncture point nerve stimulator (Beijing Huawei Co. Ltd., Beijing, China). The stimulation was used at a current of 3–5 mA, depending on the subject’s tolerance, with a low frequency (2 Hz). In the sham group, the same acupuncture needles that were used in the EA group were placed at similar acupoints and were secured by adhesive tape without penetrating the skin [11]. The electrodes were also connected but no electronic current was applied.

In our hospital, intravenous analgesia is generally used for postoperative pain management. The solution, which consists of 500 μg of Fentanyl and 50 mg of Flurbiprofen, was diluted with normal saline to 100 mL and was intravenously injected at a speed of 2 mL/h. In this study, the half dose of the conventional drug concentration, 250 μg of Fentanyl and 25 mg of Flurbiprofen, was used. If the visual analogue scale (VAS) scores exceeded four, despite the use of intravenous analgesia, an additional 1 mg/kg of sauteralgyl was injected intramuscularly.

2.3. Measurement

Pain intensity was assessed by a VAS score ranging from 0 (no pain) to 10 (extreme pain). A research assistant who was not involved in the patients’ EA application performed the assessments at standard time points (1 hr, 2 hr, 24 hr, 48 hr, and 72 hr after surgery). In addition to the VAS assessment, postoperative use of sauteralgyl for the first 72 hours was also recorded (mg/kg) along with other postoperative recovery data, such as the incidence of nausea and vomiting and the postoperative appearance of flatus and defecation.

The blood levels of β-EP and 5-HT were measured. Peripheral venous blood samples were collected into precooled anticoagulant tubes containing trasylol at 2 hr, 24 hr, 48 hr, and 72 hr after surgery. The plasma was separated into polyethylene tubes and was stored at −80°C until analysis. A radioimmunoassay was used to determine the plasma concentrations of β-EP. 5-HT was measured by using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Labor Diagnostika Nord, Nordhorn, Germany) according to the manufacturer’s instructions.

2.4. Data Analysis

Based on a small pilot study (six patients per group), we performed a power analysis to determine the sample size that was required to obtain significant effects for each VAS score at 24 h following surgery. We calculated that 39 patients per group would be sufficient (using power = 90% and type I error = 5) and allocated 45 per group to anticipate withdrawals. Chi-squared test was used for the comparison of proportions. One-way repeated ANOVA followed by Bonferroni’s multiple comparison test was used to compare the values between the groups at each time point. -test was applied for the comparison of other continuous measurements where appropriate. All statistical analyses were performed using SPSS software version 16.0 (SPSS Inc., Chicago, IL, USA). The level of significance for all statistical tests was set at 0.05.

3. Results

Of the 108 patients who were eligible, 92 signed the informed consent and were included in the study (Figure 1). There was no significant difference between the EA group and the sham control group with regard to baseline demographic characteristics (, Table 1).

Figure 2(a) showed the pain VAS score over time for the two groups. Before EA stimulation, the VAS scores at 1 h were not significantly different between the two groups. However, significant differences were observed at 2 hr, 24 hr, 48 hr, and 72 hr after surgery between the EA group and the sham group ( = 30.54, ). The time effect was significant in 2 hr, 24 hr, 48 hr, and 72 hr, compared to pre-EA stimulation ( = 345.31, ). The interaction effect (time × group) was also significant ( = 118.23, ), indicating time-dependent EA effects. The cumulative VAS showed significantly lower scores in the EA group compared with the sham group during the test period (, Figure 2(b)). The total dose of supplementary sauteralgyl was mg/kg in the EA group and mg/kg in the sham group (), resulting in a 20% reduction in the EA group (Figure 2(c)). Additionally, nausea was more frequent in the sham group than in the EA group (, Table 2). However, postoperative vomiting did not significantly differ between the groups (, Table 2). The time of postoperative flatus and defecation was also shorter in the EA group (, Table 2).

(a)

(b)

(c)

There were no significant differences in the β-EP and 5-HT levels between the groups at 1 hr after sugary. The β-EP level increased significantly at 2 hr, 24 hr, 48 hr, and 72 hr postoperatively in the EA group ( or ), whereas the β-EP level decreased slightly in the sham group, resulting in a significant difference between the two groups at 24 hr, 48 hr, and 72 hr postoperatively ( = 104.59, ) (Figure 3(a)). There was a sustained increase in plasma 5-HT levels postoperatively up to 72 h in the sham group ( or ) (Figure 3(b)). However, compared with the sham group, the upward trend of 5-HT levels was suppressed in the EA group at 24 hr, 48 hr, and 72 hr postoperatively ( = 9.79, ) (Figure 3(b)).

(a)

(b)

4. Discussion

Acupuncture anesthesia or acupuncture anesthesia combined with narcotic drugs has been widely reported in various types of surgeries, for example, in open heart surgery, pneumonectomy, hip replacement, hysteroscopic surgery, and nasal endoscopic sinus surgery [12–16]. These previous reports have already confirmed that perioperative EA is safe and effective in reducing perioperative analgesic requirements and postoperative morbidity [12–16].

Postthoracotomy pain is a significant issue and still remains a challenge to thoracic surgeons [17]. Many anesthetic and pharmacological agents are used to decrease postthoracotomy pain. However, these drugs carry risks of side effects and control the postthoracotomy pain effectively [18]. Furthermore, a literature search suggests that acupuncture analgesia is more efficient in surgical patients when administered postoperatively [19]. Therefore, our study aimed to determine whether postoperative EA can alleviate postoperative pain and reduce analgesic consumption and its related adverse effects.

In this study, our results indicate that postoperative EA reduced postoperative pain in thoracotomy patients compared with the sham group. Following the reduction of postthoracotomy pain, EA also decreased overall analgesic consumption by 20% in the first 72 hr postoperatively. Our results are similar to those of recent clinical trials where EA can be an effective adjunct for postsurgical pain management [20–22]. Furthermore, a systematic review found that acupuncture treatment reduced opioid consumption by 21–29% in fifteen RCTs and had clinical significance [23]. In contrast, one study showed that using implantation of small intradermal needles at acupoints did not reduce pain or analgesic requirements after thoracotomy when compared with a sham technique [24]. There could be two explanations for this controversy. First, we focused on acute postthoracotomy pain within 72 hours. However, the other study focus was on chronic postthoracotomy pain. Second, preoperative implantation of small intradermal needles was used, and the needles were retained for 4 weeks in the study [24]. This special acupuncture technique may not have delivered sufficiently strong stimulation to produce an analgesic effect [25, 26]. At present, there is a general consensus that the analgesic effect of acupuncture most likely resulted from activation of the endogenous pathway by stimulating the release of central endogenous opioid peptides β-EP and endomorphin, especially with low-frequency stimulation [27, 28]. The result of our study also supports the notion that 2 Hz EA is able to increase the plasma β-EP concentration and in turn that led to the reduction of acute postthoracotomy pain.

Nausea and vomiting are the most common side effects of opioid use. Although the frequency of postoperative vomiting was found to be similar between the two groups, the incidence of nausea in the EA group was significantly lower than that in the sham group during the 72 hr postoperative period. This observation may be caused by the fact that patients in the EA group have required less amount of postoperative analgesia than the sham group and/or the lower plasma level of 5-HT in EA group than in sham group. As 5-HT is one of the key modulators that regulate the motility of gastrointestinal tract, EA may regulate the release of 5-HT and resulted in faster returning of bowel function [29], as demonstrated in our results shortening the time to first flatus and defecation. This may also be the less analgesic consumption, hence less chance for the incidence of nausea.

Although there are different central mechanisms between acupoints and nonacupoints, the penetration of a needle through the skin can produce a physiological effect [30]. Furthermore, even with the same stimulus mode, different acupoints have different responses in the brain and different therapeutic effects [31, 32]. To exclude the placebo effect, the sham group used similar acupoints in this study. In some cases, however, there was no obvious placebo effect [20]. There are several limitations in our current study. First, we did not include a “control” group in our study design. However, a previous study suggested that the sham group is not superior to the control group [20]. Second, we did not record pain score during activities.

In conclusion, postoperative EA is advantageous in reducing acute postoperative pain in the first 72 hours postoperatively, analgesic consumption, incidence of nausea, and the time to first flatus and defecation. Thus, EA is an effective adjunctive therapy for postoperative pain management in patients undergoing thoracotomy. Future studies will focus on whether postoperative EA improves patient’s recovery and prevents the development of chronic thoracotomy pain syndrome.

Competing Interests

The authors declare no conflict of interests, financial or otherwise.

Authors’ Contributions

Jia Zhou and Tongyu Chen designed the conception. TongyuChen, Jianjun Xu, and Wen Ma conducted the trials. Ke Wang analyzed data. Tongyu Chen, Ke Wang, and Jia Zhou interpreted the results and prepared figures. Ke Wang drafted the paper. Ke Wang, Tongyu Chen, and Jia Zhou edited and revised the paper. Tongyu Chen, Ke Wang, Jianjun Xu, Wen Ma, and Jia Zhou approved the final version of the paper.

Acknowledgments

This study was supported in part by the National Basic Research Program of China (no. 973-2013CB531901), the National Natural Science Foundation of China (Grant no. 81202767), Research Grant from Shanghai Hospital Development Center (no. SHDC12013120), Science and Technology Support Program of Science and Technology Commission of Shanghai Municipality (no. 12401903200), Medical Guide Project of Shanghai Municipal Science and Technology Commission (no. 12401905900), and Development of Chinese Medicine in Shanghai Three-Year Action Plan (no. ZYSNXD-CC-ZDYJ 014). The authors give their thanks to all the staff of Ward 4A and ICU for this work.

References

Z. Peng, H. Li, C. Zhang, X. Qian, Z. Feng, and S. Zhu, “A retrospective study of chronic post-surgical pain following thoracic surgery: prevalence, risk factors, incidence of neuropathic component, and impact on qualify of life,” PLoS ONE, vol. 9, no. 2, Article ID e90014, 2014.
View at: Publisher Site | Google Scholar
K. Wildgaard, J. Ravn, and H. Kehlet, “Chronic post-thoracotomy pain: a critical review of pathogenic mechanisms and strategies for prevention,” European Journal of Cardio-Thoracic Surgery, vol. 36, no. 1, pp. 170–180, 2009.
View at: Publisher Site | Google Scholar
E. A. Ochroch and A. Gottschalk, “Impact of acute pain and its management for thoracic surgical patients,” Thoracic Surgery Clinics, vol. 15, no. 1, pp. 105–121, 2005.
View at: Publisher Site | Google Scholar
A. Fiorelli, G. Vicidomini, P. Laperuta et al., “Pre-emptive local analgesia in video-assisted thoracic surgery sympathectomy,” European Journal of Cardiothoracic Surgery, vol. 37, no. 3, pp. 588–593, 2010.
View at: Publisher Site | Google Scholar
C. Y. Tzeng, S. L. Chang, C. C. Wu et al., “Single-blinded, randomised preliminary study evaluating the effects of 2 Hz electroacupuncture for postoperative pain in patients with total knee arthroplasty,” Acupuncture in Medicine, vol. 33, no. 4, pp. 284–288, 2015.
View at: Publisher Site | Google Scholar
V. Ntritsou, C. Mavrommatis, C. Kostoglou et al., “Effect of perioperative electroacupuncture as an adjunctive therapy on postoperative analgesia with tramadol and ketamine in prostatectomy: a randomised sham-controlled single-blind trial,” Acupuncture in Medicine, vol. 32, no. 3, pp. 215–222, 2014.
View at: Publisher Site | Google Scholar
M. Iacobone, M. Citton, S. Zanella et al., “The effects of acupuncture after thyroid surgery: a randomized, controlled trial,” Surgery, vol. 156, no. 6, pp. 1605–1613, 2014.
View at: Publisher Site | Google Scholar
R. H. L. Wong, T. W. Lee, A. D. L. Sihoe et al., “Analgesic effect of electroacupuncture in postthoracotomy pain: a prospective randomized trial,” Annals of Thoracic Surgery, vol. 81, no. 6, pp. 2031–2036, 2006.
View at: Publisher Site | Google Scholar
S.-X. Ma, “Neurobiology of acupuncture: toward CAM,” Evidence-Based Complementary and Alternative Medicine, vol. 1, no. 1, pp. 41–47, 2004.
View at: Publisher Site | Google Scholar
F. Qu and J. Zhou, “Electro-acupuncture in relieving labor pain,” Evidence-Based Complementary and Alternative Medicine, vol. 4, no. 1, pp. 125–130, 2007.
View at: Publisher Site | Google Scholar
M. Takayama, H. Yajima, A. Kawase, I. Homma, M. Izumizaki, and N. Takakura, “Is skin-touch sham needle not placebo? A double-blind crossover study on pain alleviation,” Evidence-Based Complementary and Alternative Medicine, vol. 2015, Article ID 152086, 7 pages, 2015.
View at: Publisher Site | Google Scholar
W. Ma, Y.-M. Zhu, H. Zhou, G.-Q. Fu, H. Pan, and W.-D. Shen, “Protecting action of acupuncture-drug compound anesthesia with different frequency electroacupuncture on stress reaction in pneumonectomy,” Zhongguo Zhen Jiu, vol. 31, no. 11, pp. 1020–1024, 2011.
View at: Google Scholar
Q.-Y. Tong, W. Ma, W.-D. Shen, Z.-J. Zhang, and C. Zhao, “Analgesic effect of acupuncture compound anesthesia in functionality nasal endoscopic sinus surgery,” Zhongguo Zhen Jiu, vol. 32, no. 9, pp. 815–818, 2012.
View at: Google Scholar
H. Yang, X.-Q. Yin, G.-A. Li, L. Yuan, and H. Zhou, “Effect of application of acupuncture-anesthetic composite anesthesia on hysteroscopic surgery: a clinical study,” Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi, vol. 34, no. 7, pp. 804–807, 2014.
View at: Google Scholar
L. Yuan, W. Tang, J. Wang, and G.-Q. Fu, “Effects of transcutaneous electrical acupoint stimulation combined with general anesthesia on cerebral oxygen metabolism in elderly hip replacement patients during controlled hypotension,” Zhen Ci Yan Jiu, vol. 39, no. 1, pp. 11–19, 2014.
View at: Google Scholar
J. Zhou, H. Chi, T. O. Cheng et al., “Acupuncture anesthesia for open heart surgery in contemporary China,” International Journal of Cardiology, vol. 150, no. 1, pp. 12–16, 2011.
View at: Publisher Site | Google Scholar
R. G. Soto and E. S. Fu, “Acute pain management for patients undergoing thoracotomy,” Annals of Thoracic Surgery, vol. 75, no. 4, pp. 1349–1357, 2003.
View at: Publisher Site | Google Scholar
R. Sapkota, U. K. Shrestha, and P. Sayami, “Intercostal muscle flap and intracostal suture to reduce post-thoracotomy pain,” Asian Cardiovascular and Thoracic Annals, vol. 22, no. 6, pp. 706–711, 2014.
View at: Publisher Site | Google Scholar
S.-M. Wang, Z. N. Kain, and P. F. White, “Acupuncture analgesia. II. Clinical considerations,” Anesthesia and Analgesia, vol. 106, no. 2, pp. 611–621, 2008.
View at: Publisher Site | Google Scholar
Y.-C. Chung, M.-Y. Tsou, H.-H. Chen, J.-G. Lin, and M.-L. Yeh, “Integrative acupoint stimulation to alleviate postoperative pain and morphine-related side effects: a sham-controlled study,” International Journal of Nursing Studies, vol. 51, no. 3, pp. 370–378, 2014.
View at: Publisher Site | Google Scholar
H. Wang, Y. Xie, Q. Zhang et al., “Transcutaneous electric acupoint stimulation reduces intra-operative remifentanil consumption and alleviates postoperative side-effects in patients undergoing sinusotomy: a prospective, randomized, placebo-controlled trial,” British Journal of Anaesthesia, vol. 112, no. 6, pp. 1075–1082, 2014.
View at: Publisher Site | Google Scholar
B. J. He, P. J. Tong, J. Li, H. T. Jing, and X. M. Yao, “Auricular acupressure for analgesia in perioperative period of total knee arthroplasty,” Pain Medicine, vol. 14, no. 10, pp. 1608–1613, 2013.
View at: Publisher Site | Google Scholar
Y. Sun, T. J. Gan, J. W. Dubose, and A. S. Habib, “Acupuncture and related techniques for postoperative pain: a systematic review of randomized controlled trials,” British Journal of Anaesthesia, vol. 101, no. 2, pp. 151–160, 2008.
View at: Publisher Site | Google Scholar
G. Deng, V. Rusch, A. Vickers et al., “Randomized controlled trial of a special acupuncture technique for pain after thoracotomy,” Journal of Thoracic and Cardiovascular Surgery, vol. 136, no. 6, pp. 1464–1469, 2008.
View at: Publisher Site | Google Scholar
S. Hantoushzadeh, N. Alhusseini, and A. H. Lebaschi, “The effects of acupuncture during labour on nulliparous women: a randomised controlled trial,” Australian and New Zealand Journal of Obstetrics and Gynaecology, vol. 47, no. 1, pp. 26–30, 2007.
View at: Publisher Site | Google Scholar
S. Shukla, A. Torossian, J.-R. Duann, and A. Leung, “The analgesic effect of electroacupuncture on acute thermal pain perception—a central neural correlate study with fMRI,” Molecular Pain, vol. 7, article 45, 2011.
View at: Publisher Site | Google Scholar
D. Mayor, “An exploratory review of the electroacupuncture literature: clinical applications and endorphin mechanisms,” Acupuncture in Medicine, vol. 31, no. 4, pp. 409–415, 2013.
View at: Publisher Site | Google Scholar
J.-S. Han, “Acupuncture: neuropeptide release produced by electrical stimulation of different frequencies,” Trends in Neurosciences, vol. 26, no. 1, pp. 17–22, 2003.
View at: Publisher Site | Google Scholar
C. C. Horn, W. J. Wallisch, G. E. Homanics, and J. P. Williams, “Pathophysiological and neurochemical mechanisms of postoperative nausea and vomiting,” European Journal of Pharmacology, vol. 722, no. 1, pp. 55–66, 2014.
View at: Publisher Site | Google Scholar
M. Yang, J. Yang, F. Zeng et al., “Electroacupuncture stimulation at sub-specific acupoint and non-acupoint induced distinct brain glucose metabolism change in migraineurs: a PET-CT study,” Journal of Translational Medicine, vol. 12, article 351, 2014.
View at: Publisher Site | Google Scholar
S.-P. Kong, Q.-W. Tan, Y. Liu et al., “Specific correlation between the Hegu point (LI4) and the orofacial part: evidence from an fMRI study,” Evidence-Based Complementary and Alternative Medicine, vol. 2015, Article ID 585493, 7 pages, 2015.
View at: Publisher Site | Google Scholar
S. J. Wang, H. Y. Yang, F. Wang, and S. T. Li, “Acupoint specificity on colorectal hypersensitivity alleviated by acupuncture and the correlation with the brain–gut axis,” Neurochemical Research, vol. 40, no. 6, pp. 1274–1282, 2015.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2016 Tongyu Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

1872

Downloads

1299

Citations