Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Figure 4

HPLC chromatograms of ethyl acetate fraction from Flos Chrysanthemi Indici and HSCCC peak fractions. (A) Ethyl acetate fraction from Flos Chrysanthemi Indici; (1–4) purified HSCCC peak fractions 1–4 in Figure 3. Conditions: column, Boston crest C18, (250 mm × 4.6 mm, ID 5 μm); column temperature, 25°C; mobile phase, acetonitrile, and 0.1% H₃PO₄ aq at the gradient (acetonitrile 0–14 min, 16–21%; 14–32 min, 21–34%; 32–42 min, 34–42%; 42–60 min, and 42–80 min); flow rate, 1.0 mL/min; detection, 326 nm.