Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Figure 8

Effect of the four compounds on mineralization of MC3T3-E1 cells. (a) After treatment with test agent at concentration of 1 μM for 21 days, the calcium deposition was assessed by Alizarin red-S staining (×4). (b) Calcium concentration analysis demonstrated that the four compounds increased the mineralization of MC3T3-E1 cells. Data are represented as the means ± SD of three independent experiments. as compared with the control.

(a)

(b)