Role of the ER/NO/cGMP Signaling Pathway in the Promotion of Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Actaea racemosa Extract

Table 1

Primer sequences for real-time fluorescence-based quantitative PCR.

Gene	Genomic library code	Primer sequence	Sequence length/bp
eNOS	NM_021838.2	Forward 5′-ACTATGGCAACCAGCGTCCT-3′	143
		Reverse 5′-CGCAATGTGAGTCCGAAAATG-3′
iNOS	NM_012611.3	Forward 5′-CTCACTGTGGCTGTGGTCACCTA-3′	101
		Reverse 5′-GGGTCTTCGGGCTTCAGGTTA-3′
ALP	NM_013059.1	Forward 5′-TGGTACTCGGACAATGAGATGC-3′	219
		Reverse 5′-GCTCTTCCAAATGCTGATGAGGT-3′
β-Actin	NM_031144	Forward 5′-TGCTATGTTGCCCTAGACTTCG-3′	240
		Reverse 5′-GTTGGCATAGAGGTCTTTACGG-3′