Research Article
Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway
Figure 3
The control groups included MCF-7 cells cultured in DMEM with 10−8 M E2 and cells cultured in the absence of E2. When treated with SSCE (80 μg/ml, 160 μg/ml, and 320 μg/ml) in the presence of 10−8 M E2, the proportion of cells in the G1 phase of the cell cycle was exacerbated. The proportion of cells in the S phase was reduced in SSCE treated groups. There was no difference in the proportion of cells in the G2 phase between these groups. The cells were cultured in phenol red-free DMEM with charcoal-stripped FBS.