Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Figure 5

(a) The activity of nucleus ERE, p-ERα, and p-ERβ in MCF-7 cells cultured without estrogen detected by a double-fluorescence enzyme reporting system is shown. Relative to ERE, the activation of p-ERα and p-ERβ in the control group was significantly higher than that in MCF-7 cells cultured with SSCE (160 μg/ml and 320 μg/ml) for 12 h. (b) p-ERα and p-ERβ protein levels after treatment with SSCE (80 μg/ml, 160 μg/ml, and 320 μg/ml) cultured for 24 h were detected by Western blot. SSCE inhibited p-ERα expression in a concentration-dependent manner. However, SSCE did not affect the expression of p-ERβ.

(a)

(b)