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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 3064072, 5 pages
Research Article

Ultrastructure of a Mobile Threadlike Tissue Floating in a Lymph Vessel

1Nano Primo Research Center, Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Republic of Korea
2Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, Republic of Korea
3Integrative Research Support Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
4Department of Acupuncture & Moxibustion, Wonkwang University, Gwangju Medical Hospital, Gwangju 503-310, Republic of Korea

Received 29 October 2015; Revised 11 February 2016; Accepted 15 February 2016

Academic Editor: Morry Silberstein

Copyright © 2016 Sharon Jiyoon Jung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Observations of the primo vascular system (PVS) floating in lymph ducts were reported by various groups. There have been, however, no studies on the ultrastructure of the entire cross section of a primo vessel (PV) inside a lymph vessel with a transmission electron microscope (TEM). In the current study we took the TEM images of a cross section of the PV inside a lymph vessel. We used the Alcian blue staining method for the finding of the target PV in a lymphatic vessel by injecting the dye into the inguinal lymph nodes. The stained PV was harvested together with the lymph vessel and some parts of the specimens were used for studying with optical microscopes. Some other parts were treated according to a standard protocol for TEM. As the results the TEM study revealed the loosely distributed collagen fibers with plenty of empty spaces and the lumens with the endothelial nuclei. It turned out to be very similar to the ultrastructure of the PVs observed on the surfaces of internal organs. It also showed how compactly the PV is surrounded with lymphocytes. In conclusion, the detailed morphological features like the distribution of fibers in the PV were revealed and shown to be similar to another kind of the PV on the surfaces of internal organs.