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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 3124519, 10 pages
http://dx.doi.org/10.1155/2016/3124519
Research Article

Pinoresinol Diglucoside Alleviates oxLDL-Induced Dysfunction in Human Umbilical Vein Endothelial Cells

1Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China
2Department of Cardiology, Yantai Yeda Hospital of Binzhou Medical University, Yantai, Shandong 264100, China
3Department of Geriatrics, Yantai Yeda Hospital of Binzhou Medical University, Yantai, Shandong 264100, China
4Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, China

Received 18 July 2016; Revised 11 October 2016; Accepted 19 October 2016

Academic Editor: Yong C. Boo

Copyright © 2016 Jinpeng Yao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Atherosclerotic cardiovascular diseases are the leading causes of morbidity and mortality worldwide. Deposition of oxidized low-density lipoprotein (oxLDL) is one of the initiators and promoters of atherosclerosis. Eucommia lignans were shown to possess antihypertensive effects. This study aimed to investigate the effects of pinoresinol diglucoside (PD), a Eucommia lignan, on oxLDL-induced endothelial dysfunction. HUVECs were treated with oxLDL and/or PD followed by assessing radical oxygen species (ROS), apoptosis, nitrogen oxide (NO), malondialdehyde (MDA), and superoxide dismutase (SOD) activity with specific assays kits, mRNA levels with quantitative real-time polymerase chain reaction (PCR), and protein levels with western blot. PD abolished oxLDL-induced ROS and MDA production, apoptosis, upregulation of lectin-like oxidized LDL recptor-1 (LOX-1), intercellular Adhesion Molecule 1 (ICAM-1), and nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB), and activation of p38MAPK (mitogen-activated protein kinases)/NF-κB signaling. Meanwhile, PD alleviated oxLDL-caused inhibition of SOD activity, eNOS expression, and NO production. These data demonstrated that PD was effective in protecting endothelial cells from oxLDL-caused injuries, which guarantees further investigation on the clinical benefits of PD on cardiovascular diseases.