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Evidence-Based Complementary and Alternative Medicine
Volume 2016 (2016), Article ID 3137486, 8 pages
Research Article

Pigmentation Effect of Rice Bran Extracted Minerals Comprising Soluble Silicic Acids

Department of Medical Biotechnology (BK21 Plus team), Dongguk University, Seoul, Republic of Korea

Received 28 July 2016; Revised 27 September 2016; Accepted 11 October 2016

Academic Editor: Gioacchino Calapai

Copyright © 2016 Hyun-Jun Jang and Young-Kwon Seo. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Our investigation focused on identifying melanogenesis effect of soluble minerals in rice bran ash extract (RBE) which include orthosilicic acid (OSA). Melanocytes were apparently normal in terms of morphology. It was, however, shown that they were stressed a little in the RBE and OSA added media in aspect of LDH activity. Melanin synthesis and intracellular tyrosinase activity were increased by treatment of RBE which is similar to that of OSA. The Western blotting results showed that TRP-1, tyrosinase, and MITF expression levels were 2-3 times higher in the OSA and RBE groups compared to the control group which promoted melanin synthesis through CREB phosphorylation. Moreover, histology and immunohistochemistry were shown to have similar result to that of protein expression. As a result, minerals which comprise orthosilicic acid has the potential to promote melanogenesis and both RBE and OSA have similar cell viability, protein expression, and immunostaining results, suggesting that RBE comprises specific minerals which promote melanin synthesis through increasing of MITF and CREB phosphorylation. Therefore, RBE could be used as a novel therapeutic approach to combat melanin deficiency related diseases by stimulating melanocytes via its soluble Si and mineral components.