Formula Compatibility Identification of Dachengqi Decoction Based on the Effects of Absorbed Components in Cerulein-Injured Pancreatic AR42J Cells

Table 1

The effects of the absorbed components from DCQD individually on cerulein-induced AR42J cell death and LDH release.

Component	Cell viability (%)	LDH release (%)
Rhein	76.02 ± 1.32	20.73 ± 0.78
Emodin	73.05 ± 1.73	24.15 ± 0.67
Chrysophanol	73.05 ± 1.73	22.44 ± 0.47
Rheochrysidin	72.76 ± 2.04	24.87 ± 1.03
Aloe-emodin	71.38 ± 1.58	25.73 ± 0.84
Naringin	72.48 ± 1.02	26.82 ± 0.96
Hesperidin	69.60 ± 1.64	29.63 ± 1.21
Naringenin	68.40 ± 1.77	33.04 ± 0.57
Honokiol	75.04 ± 1.64	21.71 ± 0.66
Magnolol	73.35 ± 2.11	22.68 ± 0.75
Cerulein alone	63.47 ± 1.03	40.24 ± 2.03
Normal	92.30 ± 1.32	5.31 ± 0.23

Rhein, emodin, chrysophanol, rheochrysidin and aloe-emodin are from Dahuang, naringenin, naringin and hesperidin are from Zhishi, and magnolol and honokiol are from Houpo. LDH = lactate dehydrogenase. Cells were pretreated with the ten components with the peak concentrations for 30 min and then coincubated with 10 nM cerulein for 24 h. After cerulein added, cell viability examined by WST-8 assay. Necrotic cell death was assessed by the release of LDH from the cytosol of damaged cells into the supernatant using the LDH Cytotoxicity Detection Kit. The results are mean ± SD. versus rhein-treated group, versus naringin-treated group; versus honokiol-treated group.