Chinese Herbal Mixture, Tien-Hsien Liquid, Induces G2/M Cycle Arrest and Radiosensitivity in MCF-7 Human Breast Cancer Cells through Mechanisms Involving DNMT1 and Rad51 Downregulation

<div>THL inhibited AKT and ERK signaling pathways in MCF-7 cells. (a) (A) THL decreased both the phosphorylated (p-AKT) and total AKT proteins in a dose-dependent manner. (B) THL decreased phosphorylated ERK protein (p-ERK) at doses above 1.5 mg/mL but did not affect the total ERK protein level. Cells were treated with PBS or THL for 72 h and then harvested. Cell lysates were then prepared for Western blot analysis. (b) The blots were normalized to actin or tubulin, and the fold change protein level expression is shown in comparison to PBS-treated control.</div>

Evidence-Based Complementary and Alternative Medicine

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Figure 5

Figure 5: Chinese Herbal Mixture, Tien-Hsien Liquid, Induces G2/M Cycle Arrest and Radiosensitivity in MCF-7 Human Breast Cancer Cells through Mechanisms Involving DNMT1 and Rad51 Downregulation 

Figure 5 | Chinese Herbal Mixture, Tien-Hsien Liquid, Induces G2/M Cycle Arrest and Radiosensitivity in MCF-7 Human Breast Cancer Cells through Mechanisms Involving DNMT1 and Rad51 Downregulation 