Figure 1: The effects of eGrs on the RANKL-induced osteoclast differentiation. (a) BMMs were cultured for 4-5 days in the presence of M-CSF (10 ng/mL), RANKL (20 ng/mL), and various concentrations of eGr. Osteoclasts were stained with TRAP. (b) The TRAP-positive MNCs containing three or more nuclei were scored as osteoclasts. (c) TRAP activity was measured at 405 nm. Results are represented as means ± SD of three independent experiments. , significant differences from the control.