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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 3430405, 8 pages
http://dx.doi.org/10.1155/2016/3430405
Research Article

DNA Protection against Oxidative Damage Using the Hydroalcoholic Extract of Garcinia mangostana and Alpha-Mangostin

1Laboratório de Biologia de Fungos, Centro de Biotecnologia, Universidade Estadual de Santa Cruz (UESC), Rodovia Jorge Amado, km 16, 45662900 Ilhéus, BA, Brazil
2Laboratório de Farmacogenômica e Epidemiologia Molecular, Universidade Estadual de Santa Cruz (UESC), Rodovia Jorge Amado, km 16, 45662900 Ilhéus, BA, Brazil
3Laboratório de Desenvolvimento de Vacinas, Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo, São Paulo, SP, Brazil
4Genotox-Royal, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves, 9500/Setor 4-Pred 43.421/S113, 91501970 Porto Alegre, RS, Brazil
5Laboratório de Imunofarmacologia, Universidade Federal de Mato Grosso (UFMT), Avenida Fernando Corrêa da Costa, n° 2367, Boa Esperança, 78060900 Cuiabá, MT, Brazil

Received 27 October 2015; Accepted 31 January 2016

Academic Editor: Rainer W. Bussmann

Copyright © 2016 Ronaldo Carvalho-Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Garcinia mangostana, popularly known as “mangosteen fruit,” originates from Southeast Asia and came to Brazil about 80 years ago where it mainly grows in the states of Pará and Bahia. Although mangosteen or its extracts have been used for ages in Asian folk medicine, data on its potential genotoxicity is missing. We, therefore, evaluated genotoxicity/mutagenicity of hydroethanolic mangosteen extract [HEGM, 10 to 640 μg/mL] in established test assays (Comet assay, micronucleus test, and Salmonella/microsome test). In the Comet assay, HEGM-exposed human leukocytes showed no DNA damage. No significant HEGM-induced mutation in TA98 and TA100 strains of Salmonella typhimurium (with or without metabolic activation) was observed and HEGM-exposed human lymphocytes had no increase of micronuclei. However, HEGM suggested exposure concentration-dependent antigenotoxic potential in leukocytes and antioxidant potential in the yeast Saccharomyces cerevisiae. HEGM preloading effectively protected against H2O2-induced DNA damage in leukocytes (Comet assay). Preloading of yeast with HEGM for up to 4 h significantly protected the cells from lethality of chronic H2O2-exposure, as expressed in better survival. Absence of genotoxicity and demonstration of an antigenotoxic and antioxidant potential suggest that HEGM or some substances contained in it may hold promise for pharmaceutical or nutraceutical application.