DNA Protection against Oxidative Damage Using the Hydroalcoholic Extract of Garcinia mangostana and Alpha-Mangostin

Figure 4

Antigenotoxicity of alpha-mangostin measured by the amount of DNA damage detected by the Comet assay. Human leukocytes were treated (preloaded) with 12.5 μM alpha-mangostin for 4 h and then exposed for 5 minutes to different concentrations of H₂O₂ (1, 0.5 and 0.25 mM) at 37°C. (a) Positive control, 1 mM H₂O₂; (b, c, and d) H₂O₂ exposure of 1, 0.5, and 0.25 mM, respectively; ethanol as negative control. (a) versus (b, c, and d) ; (c) versus (d) ().