Jin Fu Kang Oral Liquid Inhibits Lymphatic Endothelial Cells Formation and Migration

Figure 3

LECs with positive LYVE-1 immunostaining differentiated from CD34⁺/VEGFR-3⁺ EPCs. The CD34⁺/VEGFR-3⁺ cells were cultured in the presence of SDF-1 (100 ng/mL) and VEGF-C (60 ng/mL). The cells expressed LYVE-1 marker after induction with no addition ((a), control), SDF-1 (b), VEGF-C (c), and SDF-1 + VEGF-C (d) for two weeks. Integral optical density (IOD) of LYVE-1 fluorescence of each group (e). Values are expressed as mean ± SD, . , significant increase versus control group.

(a)

(b)

(c)

(d)

(e)