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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 3689208, 14 pages
Research Article

Glucotoxicity Induced Oxidative Stress and Inflammation In Vivo and In Vitro in Psammomys obesus: Involvement of Aqueous Extract of Brassica rapa rapifera

1Laboratory of Physiology of Organisms, Team of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, University of Technological Sciences Houari Boumediene, BP 32, EL Alia, 16011 Algiers, Algeria
2Laboratory of Organic and Functionally Analysis, Faculty of Chemistry, USTHB, BP 32, El Alia, 16011 Algiers, Algeria
3Laboratory of Nuclear Medicine of Central Hospital of Army, Ain Naadja, 16005 Algiers, Algeria
4Team of Biochemistry and Extracellular Matrix Remodeling, Faculty of Biological Sciences, USTHB, BP 32, El Alia, 16011 Algiers, Algeria
5Laboratory of Biochemistry of Central Hospital of Army, Ain Naadja, 16005 Algiers, Algeria

Received 27 October 2015; Revised 29 November 2015; Accepted 26 January 2016

Academic Editor: Vincenzo De Feo

Copyright © 2016 Sihem Berdja et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Context. Brassica rapa is considered as natural source of antioxidants and is used to treat diabetes. Objective. Our study carried the impact of glucotoxicity induced in vivo and in vitro in vascular smooth muscle cells (VSMCs) in Psammomys and the therapeutic effect of Brassica rapa (AEBr). Materials and Methods. We administered a hyperglucidic diet (30% sucrose) for 9 months and a treatment for 20 days with AEBr at 100 mg/kg. VSMCs were submitted to D-Glucose (0.6%) for 48 hours and treated with AEBr (2100 μg/mL) for 24 hours. We measured, in blood metabolic parameters, the redox statues and inflammatory markers in adipose tissue. Histological study was effectuated in liver. In VSMCs, we measured markers of glucotoxicity (IRS1p Serine, AKT) inflammation (NO, MCP1, TNFα, and NF-κB) and oxidative stress (oxidants and antioxydants markers). Cell viability and apoptosis were estimated by the morphological study. Results. AEBr corrects the metabolic parameters and inflammatory and oxidative markers in blood and homogenate tissue and reduces lipid droplets in liver. It induces, in VSMCs, a significant decrease of IRS1p serine, cyt c, NO, MCP1, TNFα, NF-κB, protein, and lipid oxidation and increases cell viability, AKT, ERK1/2, catalase, and SOD activity. Conclusion. Brassica enhanced the antidiabetic, anti-inflammatory, and antioxidant defense leading to the protection of cardiovascular diseases.