Identification of Plants That Inhibit Lipid Droplet Formation in Liver Cells: Rubus suavissimus Leaf Extract Protects Mice from High-Fat Diet-Induced Fatty Liver by Directly Affecting Liver Cells

Figure 4

Effect of RSE on intracellular TG content in Hepa 1–6 cells. (a) TG content in RSE-treated Hepa 1–6 cells. Dried Rubus suavissimus leaves were ground with a mill and 0.05 g of the resulting powder was extracted with 500 μL of water at 60°C for 1 h. This extract was considered to be 100% concentrated. TG in RSE-treated (RSE 5% and 7% final concentrations in the medium) and untreated Hepa 1–6 cells (RSE 0%) were analyzed by silica-gel TLC. The number of cells corresponding to 150 μg protein was used for the extraction of total lipids and their analysis. After separation by TLC, lipids were visualized with cupric acetate reagent. TG (olive oil) and oleic acid standards of the indicated amounts are shown. (b) TG contents were quantitated after separation by TLC. Data are means of three independent experiments presented with SD bars. Details are explained in Section 2.

(a)

(b)