Identification of Plants That Inhibit Lipid Droplet Formation in Liver Cells: Rubus suavissimus Leaf Extract Protects Mice from High-Fat Diet-Induced Fatty Liver by Directly Affecting Liver Cells

Figure 8

Histological detection of mouse NAFLD. Frozen sections of liver were prepared and after fixation, intracellular lipid droplets were stained red with Oil Red O. Nuclei were stained blue with hematoxylin staining to identify individual cells. Livers from a mouse fed a normal diet and mice fed a high-fat diet with (HFD + RSE) or without RSE (HFD) are shown. In the liver sections of HFD-fed mice, many red-stained lipid droplets were observed, whereas only a small number of lipid droplets were seen in the livers of mice fed a normal diet. The number and size of lipid droplets were much lower in the livers of RSE-treated mice (HFD + RSE).