Evidence-Based Complementary and Alternative Medicine / 2016 / Article / Fig 2

Research Article

Antibacterial and Anti-Inflammatory Activities of Physalis Alkekengi var. franchetii and Its Main Constituents

Figure 2

Effect of 50-EFP on LPS-induced TNF-α, IL-1β, and IL-6 cytokine production. (a) THP-1 cells were pretreated with 50-EFP for 6 h before being incubated with LPS for 24 hours. Production of TNF-α, IL-1β, and IL-6 cytokine was measured by ELISA. (b, c) Effect of 50-EFP on translocation of the NF-κB (p65) subunit into the nucleus and release of I-κBα into the cytosol upon LPS stimulation. The cells were treated with LPS alone or with LPS and 50-EFP for 6 hours. The level of I-κBα protein in the cytosol and NF-κB (p65) protein present in the cytosol and nucleus was determined by the Western blot analysis using anti-I-κBα or anti-NF-κB (p65) antibody. β-actin and TBP were used for cytosolic and nuclear control protein, respectively (in relative protein density units). versus control, ; versus LPS only group. Data are representative of three independent experiments.
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