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Evidence-Based Complementary and Alternative Medicine
Volume 2016, Article ID 4541580, 10 pages
Research Article

Decoction and Fermentation of Selected Medicinal Herbs Promote Hair Regrowth by Inducing Hair Follicle Growth in Conjunction with Wnts Signaling

1College of Life Science, Gangneung-Wonju National University, 120 Gangneung Daehangno, Gangneung, Gangwon 210-702, Republic of Korea
2College of Pharmacy, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea
3Industry-Academic Cooperation Foundation, Hanbat National University, Daejeon 305-719, Republic of Korea
4Heo-jong Oriental Medicine Clinic, Mia-dong, Gangbuk-gu 62-3, Seoul 01205, Republic of Korea

Received 26 November 2015; Revised 25 January 2016; Accepted 7 February 2016

Academic Editor: Jian-Li Gao

Copyright © 2016 Su Kil Jang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


It is well recognized that regulating the hair follicle cycle in association with Wnt signaling is one of the most interesting targets for promoting hair regrowth. In this study, we examined whether selected herbal medicines processed by decoction and fermentation promote hair growth by upregulating the number and size of hair follicles and Wnt signaling, including activation of β-catenin and Akt in telogen-synchronized C57BL/6N mice. The results revealed that the fermented extract after decoction (FDE) more effectively promoted hair growth than that of a nonfermented extract (DE). Notably, FDE effectively enhanced formation of hair follicles with clearer differentiation between the inner and outer root sheath, which is observed during the anagen phase. Mechanistic evidence was found for increased β-catenin and Akt phosphorylation levels in dorsal skin tissue along with elevated expression of hair regrowth-related genes, such as Wnt3/10a/10b, Lef1, and fibroblast growth factor 7. In conclusion, our findings suggest that FDE plays an important role in regulating the hair cycle by increasing expression of hair regrowth-related genes and activating downstream Wnt signaling targets.