Gigantol Inhibits Epithelial to Mesenchymal Process in Human Lung Cancer Cells

Figure 5

The effect of gigantol on EMT regulating proteins. (a) After H460 cells were treated with noncytotoxic doses of gigantol (0–20 μM), the expression of β-catenin, phosphorylated AKT (Ser473), AKT, phosphorylated GSK-3β (Ser9), and GSK-3β was evaluated using Western blot assay. (b) AKT inhibitor, perifosine, was used to treat H460 cells (0–10 μM); the expression of β-catenin, phosphorylated AKT (Ser473), AKT, phosphorylated GSK-3β (Ser9), GSK-3β, and Slug was evaluated using Western blot assay. The blots were reprobed with GAPDH to confirm equal loading. The immunoblot signals were qualified by densitometry. The data represent mean ± SE (). versus untreated control cells.

(a)

(b)