Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp of Ziziphus jujuba Mill. Cultivars in India
Table 2
Effect of fruit pulp extracts of eight cultivars of Z. jujuba and maslinic acid in protecting liver cells from CCl4 induced cytotoxicity by ameliorating oxidative stress.
Treatment
LDH units/100 mg tissue wet wt.
SOD units/100 mg tissue wet wt.
CAT units/100 mg tissue wet wt.
GR units/100 mg tissue wet wt.
Control
7.83 ± 1.16
17.83 ± 1.47
15.00 ± 1.41
0.151 ± 0.012
CCl4
43.53 ± 1.63
56.33 ± 2.25
87.5 ± 1.04
0.498 ± 0.008
AA
7.53 ± 1.47
17.5 ± 2.14
15.33 ± 1.75
0.150 ± 0.007
100 g/mL UM
7.00 ± 2.09
17.43 ± 1.72
15.65 ± 1.37
0.156 ± 0.003
100 g/mL CH
8.33 ± 1.16
17.23 ± 1.47
15.83 ± 1.22
0.159 ± 0.011
100 g/mL GO
7.17 ± 1.47
17.0 ± 1.41
15.67 ± 1.67
0.158 ± 0.006
100 g/mL SA
7.49 ± 1.13
17.60 ± 1.56
15.66 ± 1.45
0.153 ± 0.012
100 g/mL KA
7.13 ± 0.98
17.89 ± 1.23
15.78 ± 1.43
0.155 ± 0.009
100 g/mL AP
7.91 ± 1.23
17.56 ± 1.45
15.06 ± 1.72
0.153 ± 0.006
100 g/mL ME
7.55 ± 1.56
17.33 ± 1.76
15.78 ± 1.76
0.158 ± 0.002
100 g/mL WI
7.34 ± 1.89
17.09 ± 1.45
15.03 ± 1.29
0.156 ± 0.005
10 g/mL MA
8.01 ± 1.76
17.21 ± 1.34
15.34 ± 1.09
0.154 ± 0.006
CCl4 + 100 g/mL UM
19.83 ± 1.21
22.17 ± 1.16
25.74 ± 2.28
0.206 ± 0.008
CCl4 + 100 g/mL CH
22.74 ± 2.48
24.5 ± 0.54
28.24 ± 1.09
0.236 ± 0.008
CCl4 + 100 g/mL GO
19.33 ± 1.98
20.78 ± 0.67
22.77 ± 2.89
0.213 ± 0.004
CCl4 + 100 g/mL SA
27.0 ± 0.89
38.17 ± 1.94
36.67 ± 0.81
0.298 ± 0.008
CCl4 + 100 g/mL KA
34.33 ± 1.04
32.0 ± 0.89
44.67 ± 1.03
0.362 ± 0.008
CCl4 + 100 g/mL AP
39.33 ± 0.81
48.67 ± 0.81
47.83 ± 0.75
0.386 ± 0.005
CCl4 + 100 g/mL ME
21.33 ± 2.48
23.5 ± 0.57
27.44 ± 1.39
0.256 ± 0.008
CCl4 + 100 g/mL WI
39.33 ± 0.81
48.67 ± 0.81
47.83 ± 0.75
0.386 ± 0.055
CCl4 + 10 g/mL MA
15.83 ± 1.21
19.17 ± 1.16
23.74 ± 2.28
0.216 ± 0.003
CCl4 + 50 mM AA
13.67 ± 1.03
15.33 ± 1.63
19.83 ± 1.83
0.189 ± 0.018
Cytotoxicity was assessed in terms of % lactate dehydrogenase (LDH) released, and the response to oxidative stress was measured in terms of antioxidant enzymes. SOD: superoxide dismutase; CAT: catalase; GR: glutathione reductase activity. Ascorbic acid (AA) was used as a standard. UM: Umaran; CH: Chhuhar; GO: Gola; SA: Sannur; KA: Kadaka; AP: Apple; ME: Mehrun; WI: Wild; MA: maslinic acid. Values represent means of at least three experiments and their standard deviation. Significantly different compared with respective CCl4 treated group, . Significantly different compared with respective CCl4 treated group, .
