Quantitative Analysis of Apisin, a Major Protein Unique to Royal Jelly

Table 2

List of identified peptides corresponding to the 5 kDa protein.

Treated enzyme	Peak R.T. (min)	Sequences	Matched proteins
Lys-C	11.04	TSISVK	Apisimin (5,540 Da)
		TSLSVK	A0A088AFZ8_APIME Uncharacterized protein (26,380 Da)
Asp-N	12.60	KTSISVKGSNV	Apisimin (5,540 Da)
		KTSLSVKGESNV	Not applicable
Asp-N	15.25	DANVFA	Apisimin (5,540 Da)

These sequences were searched against the NCBI Taxonomy database (the classification “Apis”, ID: 7459).