Chinese Herbal Medicine Fuzheng Kang-Ai Decoction Inhibited Lung Cancer Cell Growth through AMPKα-Mediated Induction and Interplay of IGFBP1 and FOXO3a

Figure 3

FZKA decoction induced the IGFBP1 mRNA expression in NSCLC cells. (a) Head map compares the fold changes for genes with significantly higher (red) or lower (green) or no expression changes (black) between lung cancer cells in the presence or absence of FZKA decoction for 24 h as determined by NimbleGen Gene chip microarray analysis according to the instruction from the provider. The arrays were scanned with a Roche-NimbleGen MS200 confocal laser scanner, and the obtained images were analyzed using NimbleScan Molecular Annotation System . (b) Each point on the scatter plot graph represents a gene hybridization signal. The black points represent the ratio that ranged from 0.5 to 2.0, belonging to no different group. The red points represent the ratios that were over 2.0; the green points represent the ratios that were below 0.5. Each row corresponds to a single gene. Color represents different transcript levels, red represents higher gene expression, green represents lower gene expression, and black represents no different gene expression. (c) Total RNA was isolated from A549 cells and a quantitative real-time-PCR assay, as described in Section 2, was used for the detection and quantification of IGFBP1 transcripts. GAPDH was used as internal control. Significantly different from untreated cells .

(a)

(b)

(c)